WebAssay of β-Glucosidase β-glucosidases were assayed using p-nitrophenyl-β-D-glucopyranoside (pNPG) as substrate by the micro titer plate method (Parry et al.2001). A reaction mixture of 100 μl containing 25 μl of enzyme, 25 μl of pNPG (10 mM) as substrate, and sodium acetate buffer (50 mM, pH 5.0) was incubated at 50 oC for 30 min. WebMar 8, 2024 · The α-glycosidase enzymatic inhibition bioassay results indicated that four compounds, namely, 7, 22, 37, and 44 with representing totally different scaffolds, …
β-Glucosidase Activity Assay Kit - Sigma-Aldrich
WebMay 1, 2007 · The enzyme catalyzes the hydrolysis of p -nitrophenyl-β- d -glucopyranoside (PNPG) as the substrate, with a Km of 1.52 mM, and Vmax of 3.21 U min mg −1 protein. Glucose competitively inhibited β-glucosidase with a Ki value of 0.79 mM. Optimal activity with PNPG as the substrate was at pH 5.0 and 50°C. WebMar 8, 2024 · Increasing concentrations of substrates pNPG were used in the absence or presence of tested compounds at four different concentrations around the IC 50 values. The inhibitory kinetics of the investigated compounds on α-glucosidase was analyzed using the Lineweaver–Burk plot of the substrate concentration and velocity. Cell Viability Assay change wifi settings metered connection
Determination of α-glucosidase inhibitory activity from …
WebThe mechanism of beta-caryophyllene's pharmacological activities are not well understood, but it appears to act through the toll-like receptor 4 (TLR4). This compound also has shown promise in treating cancer cells in vitro. Beta-caryophyllene can be analyzed using chromatographic analysis or by using solid phase microextraction techniques. WebJan 15, 2024 · A simple and portable method for β-Glucosidase activity assay and its inhibitor screening based on a personal glucose meter In this study, a simple and portable … WebApr 14, 2024 · Activity assay of MWCNTs@CS@GA@α-Glu microreactor and immobilization efficiency. Study on the activity of α-glucosidase was referred to previously published literature with some modifications. Briefly, 50 μL of the uniformly dispersed MWCNTs@CS@GA@α-Glu together with 950 μL PNPG (10 mM) was mixed and vortexed … change wifi settings on kasa device