http://wolfson.huji.ac.il/purification/PDF/PAGE_SDS/Protein_Staining/PerkinElmer_PhostagBlotStain.pdf WebPolyacrylamide Gel Electrophoresis with Mn2+-Phos-tagTM-Gels 1. 5 mM Acrylamide-pendant Phos-tag™ AAL-107 (Fujifilm/ Wako) solution: dissolve 10 mg Phos-tag™ in 100 μLof ... Phosphoprotein Gel Stain 1. Plastic incubation box 12 cm 7.5 cm. 2. Pro-Q™ Diamond Phosphoprotein Gel Stain. Store at 4 Cin the dark. 3. Sodium acetate stock ...

Phospho-Tag™ Phosphoprotein Gel Stain ABP Biosciences

Webstruction Manual for Pro-Q® Diamond Phosphoprotein gel stain. Pro-Q ® Diamond Phosphoprotein Gel Stain—Tips for Success Reagent Standard Protocol Rapid Protocol Tris-glycine gels NuPAGE® Bis-Tris gels1 Tris-glycine gels NuPAGE® Bis-Tris gels2 Step 1: Fix 50% methanol, 10% acetic acid 100 mL, 30 min 2 times 100 mL, 30 min 100 mL, overnight WebThis stain contains a phospho-tag™ group, which allows direct, in-gel detection of phosphate groups attached to tyrosine, serine, or threonine residues, without the need for … east grinstead to basingstoke

Pro-Q™ Diamond Phosphoprotein Gel Stain - Thermo …

WebAug 1, 2004 · The phosphoprotein staining was performed in the cytosolic fraction using the Pro-Q Diamond phosphoprotein gel stain (Invitrogen, USA) as per manufacturer protocol [15]. The gels were... WebPhos-tag™ 540 Phosphoprotein Blot Stain (Cat. # PRD510A001KT) • Phos-tag 540 Stain Concentrate 4 vials (each 1 mL vial is enough for 4 mini-gel blots) • Phos-tag 540 Blocking Buffer (4X), 1 bottle, 100 mL • Phos-tag 540 Blot Stain Buffer (1X), 2 bottles, 200 mL each • Phos-tag 540 Wash Buffer (2X), 2 bottles, 800 mL each WebPhospho-Tag™ Phosphoprotein Gel Stain is a high sensitive fluorescent stain designed for selectively detecting phosphoproteins in polyacrylamide gels. This stain contains a … east grinstead to esher