WebSequencing and aligning of DNA gyrase encoding genes (gyrA and gyrB) and topoisomerase IV genes (parC and parE) revealed one gyrA mutation leading to the amino acid substitution Asp87Gly (Escherichia coli numbering), two gyrB mutations leading to the amino acid substitutions Ser431Pro and Met518Ile, and three parC mutations leading to … WebThe ParE protein is a toxin that inhibits DNA gyrase activity and thereby blocks DNA replication. Based on the Escherichia coli ParE structure, a series of linear peptides were …

RCSB PDB - 1S14: Crystal structure of Escherichia coli Topoisomerase …

Webisomerases DNA gyrase and topoisomerase IV, both of which are composed of two A and two B subunits; these subunits are encoded by the gyrA and gyrB genes for DNA gyrase and by the parC and parE genes for topoisomerase IV (8). We recently reported in vitro and in vivo fluoroquinolone-resistant mutants WebMay 22, 2024 · These are both type II topoisomerases that can cleave both DNA strands, and can thus alter DNA topology during replication or similar processes. Currently, there … gridlayoutmanager column count

Mechanism of Quinolone Action and Resistance - PMC

WebJan 26, 2024 · 7LHZ K. pneumoniae Topoisomerase IV (ParE-ParC) in complex with DNA and (3S)-10- [ (3R)-3- (1-aminocyclopropyl)pyrrolidin-1-yl]-9-fluoro-3-methyl-5-oxo-2,3 … WebJan 5, 2004 · Topoisomerase IV and DNA gyrase are related bacterial type II topoisomerases that utilize the free energy from ATP hydrolysis to catalyze topological changes in the bacterial genome. Type II topoisomerases are topoisomerases that cut both strands of the DNA helix simultaneously in order to manage DNA tangles and supercoils. They use the hydrolysis of ATP, unlike Type I topoisomerase. In this process, these enzymes change the linking number of circular DNA by ±2. … See more Type II topoisomerases increase or decrease the linking number of a DNA loop by 2 units, and it promotes chromosome disentanglement. For example, DNA gyrase, a type II topoisomerase … See more There are two subclasses of type II topoisomerases, type IIA and IIB. • Type IIA topoisomerases include the enzymes See more Strand passage Type IIA topoisomerase operates through a "two-gate" mechanism (though this is a historical notation), a mechanism supported by … See more Small molecules that target type II topoisomerase are divided into two classes: inhibitors and poisons. Due to their frequent … See more Type IIA topoisomerases are essential in the separation of entangled daughter strands during replication. This function is believed to be performed by topoisomerase II in eukaryotes and by topoisomerase IV in prokaryotes. Failure to separate these … See more Type IIA Type IIA topoisomerases consist of several key motifs: • an … See more Catenation is the process by which two circular DNA strands are linked together like chain links. This occurs after DNA replication, where two single strands are catenated and can … See more gridlayoutmanager horizontal