WebDQ Green BSA and DQ Red BSA are both provided lyo- philized, conveniently packaged in sets of five vials, each con- taining 1 mg of the protein conjugate. In the lyophilized form, … Webここでは,リソソーム内に蓄積する細胞へ供給したAgNPがリソソーム標的化BODIPY-コレステロールとDQ-BSAの蛍光を増強することを示した。 さらに,AgNPはLAMP1のサイトゾル尾部に融合したGFPの蛍光を増加させ,金属増強蛍光がリソソーム膜を通して起こることを示した。 重要なことに,AgNPを用いることにより,リソソーム動態を損傷し,変化するこ …
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WebNov 17, 2016 · The use of fluorescent DQ™-BSA in conjugation with autophagic makers and biomarkers of hybrid autophagy offers a reliable technique to monitor the formation of autolysosomes and LAPo-lysosomes ... WebFeb 1, 2024 · While developing a cell-based HCI assay to evaluate the functional capacity of the lysosome to degrade cargo by using a self-quenched BSA (DQ-BSA), we observed that MPS-IIIA MEFs experience a significant impairment in their ability to degrade DQ-BSA when compared with wild-type (WT) counterpart (Figure S1A).Thus, to identify compounds that … count the unwanted characters in the string s
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WebJun 8, 2024 · Cells grown on coverslips in 24-well plates were incubated with 10 μg/ml of DQ-BSA-Green for 12 h (37 °C, 5% CO 2), washed twice with PBS to remove excess probe and refreshed the medium. WebSep 22, 2024 · Next day, wash the cells three times with incomplete RPMI. 3. Dilute DQ-BSA-Red in complete RPMI medium to a final concentration of 10 μg/mL (stock concentration—1 mg/mL). Add 500 μL of it to the cells and incubate for 1 h at 37 °C. 4. After incubation, wash 3 times with 500 μL of incomplete RPMI. WebOct 10, 2024 · National Center for Biotechnology Information brewista coffee \u0026 hookah lounge