WebDec 31, 2002 · AmyA expression under the control of cspB promoter was assumed to be induced when raw starch was used as the sole carbon source. These results indicate that efficient simultaneous saccharification and fermentation of raw corn starch to L-lysine were achieved by C. glutamicum secreting AmyA using the cspB promoter and signal … Webadvantage of this cellular machinery, the CspB promoter and signal peptide of C. glutamicum can be used to synthesize and secrete soluble, properly folded, biologically active recombinant proteins directly into the growth medium with high purity. Since the two secretory pathways were elucidated, about 154 signal peptides have been screened.
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WebThe major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides ... a cis-element found in the promoter region of mammalian major histocompatability complex class II genes, which has the consensus sequence CTGATTGGC/TC/TAA [4,5]. The underlined penta- WebDec 1, 2011 · DNA fragments containing the promoter sequence of CspB from C. glutamicum and the signal sequence of CspA from C. ammoniagenes were fused to the CspB50TEV-Teriparatide gene. This synthesized construct was then inserted into the pPK4 plasmid and incorporated into the YDK010 bacterial strain, which is a derivative of wild … how to set up buttkicker gamer pro
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WebThe synthetic promoter library covered a wide range of strengths, ranging from 1 to 193% of the tac promoter. 68 promoters were selected and sequenced for correlation analysis between promoter ... WebFeb 6, 2012 · The latter is flanked by two loxP sites and constitutes of a transcriptional promoter (P-35S), the nptII coding sequence (CS-nptII), and a polyadenylation sequence from the nopaline synthase gene (T-nos). MON 87460 contains one copy of the insert at a single insertion site hosting both cspB and nptII intact expression cassettes. nothing bundt cake orange